Folic Acid Is Able to Polarize the Inflammatory Response in LPS Activated Microglia by Regulating Multiple Signaling Pathways

Figure 4

Signaling pathways evoked by LPS-activated BV-2 cells in folic acid-treated cells. Cells were treated with the indicated doses of folic acid (5, 10, 30, and 50 μg/mL) for 1 h before 12 h LPS (1 μg/mL) treatment. (a) Total cell lysates were prepared for a western blot analysis of the content of p-38 protein, p-ERK 1/2, and p-JNK in BV-2 cells. Protein levels were determined by measuring immunoblot band intensities by scanning densitometry: (b) p38 and p-p38; (c) ERK 1/2 p-ERK 1/2; (d) JNK and p-JNK. Data represent the means ± S.D. of five separate experiments. .

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