NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation

Figure 2

Effects of miR-99a on LPS-induced mTOR expression and NF-κB nuclear translocation. Endothelial cells were transfected with miR-99a (100 nM) mimic or rapamycin (50 nM) for 24 hours and then treated with LPS (20 μg/mL) for another 24 hours. The protein levels were detected by Western-blot. (a) The expression of different proteins analyzed by Western-blot; (b) quantified band density for mTOR relative to control, which was set as 1; (c) percentage of NF-κB in nucleus, total NF-κB presented by the summary of quantified band density for NF-κB (cytoplasm) and NF-κB (nucleus). All values are presented as mean ± SD from three independent experiments. ; versus control; ; versus LPS.

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