NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation

Figure 4

Dual luciferase reporter analysis of the regulate effects of NF-κB on miR-99a promoter plasmids. (a) The predicted NF-κB binding sites in miR-99a promoter analyzed by PROMO software. ((b), (c)) Portions of the upstream region of miR-99a cloned to create −1.5 kb, −2.0 kb, and −2.0 kb-Mut plasmids. (d) The increased p65 or p50 protein levels in HEK293 cells after being transfected with 150 ng of NF-κB subunit ORF cDNA Clones (p50, p65, or p50 + p65) in 24-well plates for 48 hours. (e) Relative luciferase activity of different promoter reporter plasmids after NF-κB overexpression. The values (mean ± SD from three independent experiments) are relative to NC-Blank, which was set as 1. ; .

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