Peroxisome Proliferator-Activated Receptor α Reduces Endothelin-1-Caused Cardiomyocyte Hypertrophy by Inhibiting Nuclear Factor-κB and Adiponectin

Figure 1

Fenofibrate inhibited ET-1-dependent hypertrophy and adiponectin expression. The role of fenofibrate and ET-1 in human cardiomyocyte cell size was assayed by pretreatment of cells with PPARα agonist (fenofibrate; FF) or PPARα antagonist (GW6471; GW) for 24 h, followed by stimulation with or without ET-1 (50 nM) for 48 h. Cells were fixed and stained with rhodamine-phalloidin (red) and DAPI (blue) followed by cell surface area quantization (scale bar, 50 μm) (a). Data are expressed as mean ± SEM of values from three independent experiments (b). ³H-leucine incorporation was determined as a measure of relative protein synthesis (c). Western blot assay and quantification of adiponectin (ADN), -myosin heavy chain (β-MHC), and B-type natriuretic peptide (BNP) expression were also demonstrated (d). , compared with untreated control group.

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