Peroxisome Proliferator-Activated Receptor α Reduces Endothelin-1-Caused Cardiomyocyte Hypertrophy by Inhibiting Nuclear Factor-κB and Adiponectin

Figure 4

Fenofibrate attenuated ET-1-induced adiponectin and NF-κB activation and DNA binding activity in cultured human cardiomyocytes. Immunofluorescence staining showed that pretreatment of HCMs with fenofibrate significantly inhibited ET-1-induced activation of adiponectin (a). The pretreatment of cells with fenofibrate (FF) inhibits adiponectin (ADN) and p65-NFκB. Histone H1 was used as a loading control for nuclear protein (b). Fenofibrate (FF) attenuates ET-1-stimulated NF-κB binding activity by EMSA assay (c). The summarized data (mean ± SEM) from 3 separate experiments were shown in the bar graph (d). , compared to untreated control group.

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