TLR Signalling Pathways Diverge in Their Ability to Induce PGE₂

Figure 1

TLR4 and TLR7-8 stimulation induce the secretion of PGE₂ by DCs. ((a) and (c)) DCs at day 6 of culture were stimulated with PAM₃CSK₄ (100 ng/mL), FSL-1 (100 ng/mL), Poly I:C (25 μg/mL), LPS (100 ng/mL), Flagellin (100 ng/mL), Imiquimod (5 μg/mL), R848 (5 μg/mL), and CpG (6 μg/mL). After 24 h, supernatants were collected and the production of PGE₂ (a) and CXCL8 (c) was evaluated by EIA or ELISA, respectively. Results are expressed as mean ± SEM (); compared with respective controls by one-way ANOVA with Dunnett’s post hoc test. (b) mRNA from DCs at day 6 of culture was extracted to analyse the expression of TLRs. Data are expressed as mean ± SEM ().

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(b)

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