TLR Signalling Pathways Diverge in Their Ability to Induce PGE₂

Figure 2

The release of PGE₂ by DCs depends on the activation of the MAPK and NF-κB pathways. (a) DCs were stimulated with increasing concentrations of LPS for 24 h and PGE₂ production was quantified by EIA. Data are expressed as mean ± SEM (); by one-way ANOVA with Dunnett’s post hoc test. (b) DCs were pretreated for 1 h with the indicated doses of U0126, PD98059, SB203580, or BAY-11-7082 and then stimulated with LPS (100 ng/mL) for 24 h. The production of PGE₂ was evaluated in cell-free supernatants by EIA. Results are expressed as mean ± SEM (); by one-way ANOVA with Dunnett’s post hoc test. (c) DCs were treated as in (b), using 1 μM of each inhibitor. The expression of COX2 and β-actin was determined by Western blot. One representative fluorogram out of three and its densitometric analysis are shown.

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