TLR Signalling Pathways Diverge in Their Ability to Induce PGE₂

Figure 5

CD11c⁺ cells produce PGE₂ in human inflamed lymph nodes. (a) Circulating mDCs were stimulated with LPS (100 ng/mL) or heat-killed E. coli (1 : 10 mDC/bacteria ratio). After 24 h, supernatants were collected and the production of PGE₂ was evaluated by EIA. Data are expressed as mean ± SEM (); by one-way ANOVA with Dunnett’s post hoc test. (b) Sections from FFPE reactive lymph nodes were stained as indicated. Cytoplasmic PGE₂ is observed in a fraction of CD11c⁺ cells. Representative double positive cells are indicated by arrow heads. Sections are counterstained with Meyer’s haematoxylin. Original magnifications: 400x (scale bar 50 μm).

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