Research Article

Adiponectin Inhibits LPS-Induced HMGB1 Release through an AMP Kinase and Heme Oxygenase-1-Dependent Pathway in RAW 264 Macrophage Cells

Figure 3

Effects of adiponectin and IL-10 on mRNA expression in RAW 264 cells. Raw 264 cells were cultured as described in Figure 1 legend and were treated with adiponectin (APN, 10 μg/mL) and IL-10 (100 ng/mL) for 18 h. RNA was extracted, and expressions of (a) TLR4, (b) TLR2, (c) MD2, (d) HMGB1, (e) IL-10, (f) HO-1, (g) Nrf2, (h) Sirt1, (i) Sirt6, and Gapdh (control) mRNAs were measured using qRT-PCR. The results are expressed as means ± SE of three independent experiments ( and significance compared with control (Cont)).