Adiponectin Inhibits LPS-Induced HMGB1 Release through an AMP Kinase and Heme Oxygenase-1-Dependent Pathway in RAW 264 Macrophage Cells

Figure 5

Effect of kinase inhibitors on adiponectin-induced HO-1 mRNA expression in RAW 264 cells. Raw 264 cells were cultured as described in Figure 1 legend and were treated with adiponectin (10 μg/mL) for 18 h in the presence of DMSO (control), compound C (10 μM), wortmannin (1 μM), or SB203580 (10 μM). RNA was extracted, and expressions of Hmox1 (HO-1) and Gapdh mRNAs were measured using qRT-PCR. The results are expressed as means ± SE of three independent experiments ( and significance compared with control).