Altered Expression of IFN-λ2 in Allergic Airway Disorders and Identification of Its Cell Origins

Figure 4

Flow cytometry analysis of expression of IFN-λ2 in dispersed human , , CD14+ (macrophages), and CD19+ (B cells) cells. Rabbit anti-human IFN-λ2 and FITC conjugated goat anti-rabbit IgG antibodies were employed to detect IFN-λ2 in cells from tonsil and lung, respectively. PE/Cy7-anti-human tryptase (AA5-PE/Cy7), anti-human chymase antibody CC4 (IgM subtype), PE conjugated rat anti-mouse IgM (CC1-PE), PE/Cy7-anti-human CD14 (CD14-PE/Cy7), and APC-anti-human CD19 (CD19-APC) antibodies were used to identify (A), (B), CD14+ (C), and CD19+ cells (D), respectively. (a) showed representative graphs of flow cytometry analysis. (b) represents boxplot analysis of the expression of IFN-λ2 in dispersed human , , CD14+, and CD19+ cells. Data are displayed as a boxplot for 6-7 human tonsil or lung tissues, which indicates the median, the interquartile range, and the largest and smallest values for the number of subjects indicated.

(a)

(b)