Indoxyl Sulfate Induces Mesangial Cell Proliferation via the Induction of COX-2

Figure 5

Effects of COX-2 specific inhibitor NS398 on cell cycle progression after IS treatment. The cells were treated with NS398 (10 μM) for 12 h before IS (500 μM) administration. (a) qRT-PCR analysis of COX-2 after COX-2 inhibitor (NS398, 10 μM, and 20 μM) treatment in MCs. (b) Western blotting analysis of COX-2 expression after COX-2 inhibitor (10 μM and 20 μM) treatment in MCs. (c–f) Representative images of cell cycle following the treatments of IS and/or COX-2 inhibitor. (g–j) Percentage of cells at G1/G0 (g), G2/M (h), S (i), and (S + G2)/M (j) phases following the treatments of IS and/or COX-2 inhibitor. Values are means ± SD; in each group.

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