Metformin Prevents Fatty Liver and Improves Balance of White/Brown Adipose in an Obesity Mouse Model by Inducing FGF21

Figure 6

Effect ofmetformin on Th17/Treg cell regulation in obese mice. (a) CD4-positive IL-17-positive (Th17) cells and CD4-positive Foxp3-positive (Treg) cells shown by immunostaining spleen tissues in untreated obese mice and metformin-treated obese mice (original magnification, ×400). (b) The populations of Th17 and Treg cells were analyzed using flow cytometry and mRNA expression was determined using real-time PCR. (c) Spleen tissues from untreated obese mice and metformin-treated obese mice were observed by immunostaining for CD4-positive, Foxp3-positive, and FGF21-positive cells. Scale bars, 10 μM (original magnification, ×400).

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