Tumor Necrosis Factor Alpha Inhibits L-Type Ca²⁺ Channels in Sensitized Guinea Pig Airway Smooth Muscle through ERK 1/2 Pathway

Table 1

Sequence of primers and PCR conditions for the different subunits of L-VDCC.

Gene	Oligonucleotides 5′-3′	Length (bp)	Amplification conditions	Reference
Ca1.1	Fw: TGGTACGTCGTCACCTCCT	237	MT = 56°C	XM_013158049
	Rv: CATCTATGATGCTGCCGATG		34 cycles
Ca1.2	Fw: AATTGCTCTGAAGATGACAGC	471	MT = 56°C	NM_001172923.1
	Rv: AGCTGCCAGGACATTGTCG		30 cycles
Ca1.3	Fw: TCCCGCCGGCAGGACTAT	459	MT = 56°C	XM_005008263
	Rv: ATCACCTTTAACCTCCCCCCA		32 cycles
Ca1.4	Fw: TACCCATCCCGGGTACCTAT	436	MT = 56°C	XM_013144287
	Rv: GAAGTGGGAGAAGATAGACT		34 cycles
GAPDH	Fw: TGAAGGTGAAGGTCGGTGTGAACG; Rv: CATGTAGGCCATGAGGTCCACCAC	~1000	MT = 56°C; 29 cycles	Chávez et al., 2013 [29]

Fw: forward primer, Rv: reverse primer, MT: melting temperature, and bp: base pairs.