C-Terminal Alpha-1 Antitrypsin Peptide: A New Sepsis Biomarker with Immunomodulatory Function

Figure 1

Incubation of neutrophil granulocytes (PMN) with CAAP48 results in cellular activation. PMN activation is shown 30 min after incubation with CAAP48 and the other AAT peptides by changes in the surface expression levels of activation/degranulation markers CD66b, CD63, CD69, and CD62L. Oxidative Burst was determined by detection of intracellular generated reactive oxygen species (oxidation of dihydrorhodamine-123 to rhodamine-123, R-123). Data obtained by using 40 µM and 100 µM peptide concentrations of one exemplary experiment out of four independent experiments are shown. Phorbol myristate acetate (PMA) was used as positive control and 1x PBS diluted in RPMI 1640 (1 : 2) as well as PMN in RPMI 1640 without PBS served as negative control.