C-Terminal Alpha-1 Antitrypsin Peptide: A New Sepsis Biomarker with Immunomodulatory Function

Figure 4

Chemotactic response of neutrophils to CAAP48 and the SNP-variant CAAP47. The chemotactic response of isolated neutrophils to CAAP48 and the SNP-variant CAAP47 was examined using disposable Boyden chambers. The number of cells migrating to the lower wells of the chamber was assessed by microscopy and using a hematological analyzer after 60 min. Control: RPMI 1640 medium alone; CAAP48 and CAAP47 were used at a concentration of 100 μM (480 ng/μL). CAAP48/CAAP47 dependent migration was normalized to spontaneous migration in controls. Each bar represents the mean ± SD of three independent experiments.