C-Terminal Alpha-1 Antitrypsin Peptide: A New Sepsis Biomarker with Immunomodulatory Function

Figure 5

MIP-1α and IL-8 concentrations after incubation of PMN with AAT peptides. The concentration of MIP-1α and IL-8 in the cell culture supernatant of PMN after peptide stimulation is shown. PMN were incubated with different AAT peptides for two and four hours at concentrations as indicated. MIP-1α and IL-8 were quantified by Bio-Plex. Each bar represents the mean ± SD of three independent experiments. The lower limit of detection for MIP-1α measurements is 1.6 pg/mL and 1.0 pg/mL for IL-8. MIP-1α and IL-8 are released in a dose-dependent manner upon PMN incubation with CAAP47 and CAAP48, while no dose-dependent MIP-1α or IL-8 release could be observed upon stimulation with VIRIP or the scrambled peptide compared to the negative control (1x PBS diluted in RPMI 1640 (1 : 2)). PMA stimulation served as positive control.