Research Article

Th17 Cell Response in Mice following Motor Nerve Injury

Figure 2

Th17 cell responses in mice. CD4+ T cells were isolated from dCLN from uninjured (Con) and axotomized WT and mice at 7 dpa ( mice/group). (a) Intracellular staining was performed for IFN-γ and IL-17 and analyzed using FACS. (b) The averaged percentages of Th1, Th17, and Th1Th17 cells in each groups were calculated and compared. (c) The averaged total number of Th1, Th17, and Th1Th17 cells in each groups was calculated on the basis of the total number of corresponding dCLN and their percentages. (d) Intracellular staining was performed for TNF-α and IL-17 and analyzed using FACS. (e) The averaged percentages of TNF-α+ and TNF-α+Th17 cells in each groups were calculated and compared. (f) The averaged total number of TNF-α+ and TNF-α+Th17 cells in each groups was calculated on the basis of the total number of corresponding dCLN and their percentages (data are presented as mean ± SD; mice/group, ; blue bars represent uninjured animals, whereas gold bars represent animals that have been subjected to axotomy).
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