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Mediators of Inflammation
Volume 2016 (2016), Article ID 6152713, 15 pages
http://dx.doi.org/10.1155/2016/6152713
Research Article

Inhibition of Extracellular Calcium Influx Results in Enhanced IL-12 Production in LPS-Treated Murine Macrophages by Downregulation of the CaMKK-AMPK-SIRT1 Signaling Pathway

1Medical Research Center, Southwest Hospital, The Third Military Medical University, Chongqing 400038, China
2Department of Pharmacology, College of Pharmacy, The Third Military Medical University, Chongqing 400038, China

Received 19 January 2016; Revised 19 April 2016; Accepted 27 April 2016

Academic Editor: Soh Yamazaki

Copyright © 2016 Xin Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary materials include one table and seven figures. In Table S1, Sequences of PCR primers was presented. Figure S1, S2 and S6 demonstrate similar effects on IL-12 production in RAW 264.7 cells as compared with murine primary peritoneal macrophages. Figure S3 describes effects of calcium deprivation on IL-12p40 production induced by LPS with or without IFN-γ. Figure S4 demonstrates that the LPS neutralizer PMB inhibited the upregulated production of IL-12 p40 and IL-6 in LPS treated murine peritoneal macrophages. Figure S5 describes that SOCE inhibitors 2-APB and LaCl3 selectively inhibited the upregulated production of IL-12p40 in LPS treated murine peritoneal macrophages. Figure S7 illustrates that the CaMKKβ inhibitor STO-609 does not affect TNF-α production in both RAW 264.7 cells and murine peritoneal macrophages.

  1. Supplementary Material