Fermented Herbal Formulas KIOM-MA128 Ameliorate IL-6-Induced Intestinal Barrier Dysfunction in Colon Cancer Cell Line

Figure 3

Effects of KIOM-MA/MA128 on Caco2 monolayer intestinal barrier function. (a) Intestinal barrier function was analyzed via the TER value, and paracellular permeability was determined using a nonabsorbable, fluorescein isothiocyanate- (FITC-) conjugated dextran probe (FD-30). Caco2 monolayer cells were pretreated with 400 μg/ml KIOM-MA/MA128 1 h prior to IL-6 (10 ng/ml and 50 ng/ml) exposure for 24 h. (b) Caco2 monolayer cells were pretreated with 400 μg/ml KIOM-MA/MA128 1 h prior to IL-6 (50 ng/ml) exposure for 24 h. ZO-1 was detected via immunofluorescence microscopy. Treatment of IL-6 caused significant disruptions of ZO-1. The ZO-1 staining was decreased in intensity and exhibited an irregular band. Four hundred μg/ml KIOM-MA128 completely prevented ZO-1 in IL-6 treated Caco2 monolayers. (c) Histograms are represented by continuous bands encircling the cells at the cellular borders. (d) Cell lysates were analyzed for ZO-1 expression via Western blot. The results are reported as the means ± SDs from 3 independent experiments. compared with the IL-6 treated group; compared with the control group.

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