Fermented Herbal Formulas KIOM-MA128 Ameliorate IL-6-Induced Intestinal Barrier Dysfunction in Colon Cancer Cell Line

Figure 4

Effects of KIOM-MA/MA128 on the mRNA and protein expression of TJ proteins in the Caco2 monolayer. Caco2 monolayer cells were pretreated with 400 μg/ml KIOM-MA/MA128 1 h prior to IL-6 (50 ng/ml) exposure for 24 h. (a) Cells were lysed, and the lysates were examined via Western blot for ZO-1, occludin, and claudin-2. Pretreatment with KIOM-MA/MA128 protected the TJ proteins. (b) Total mRNA was isolated, and the mRNA levels of TJ proteins, such as ZO-1, ZO-2, and ZO-3, occludin, and claudin-1, claudin-2, and claudin-7, were assessed. Pretreatment with KIOM-MA/MA128 protected the IL-6-induced mRNA expression. The results are reported as the means ± SDs from 3 independent experiments. compared with the IL-6 treated group; compared with the control group.