Disturbed Expression of EphB4, but Not EphrinB2, Inhibited Bone Regeneration in an In Vivo Inflammatory Microenvironment

Figure 4

Disturbed expression of EphB4, but not ephrinB2, significantly downregulated mRNA levels of bone-related genes and upregulated the mRNA level of NFATc1 in an inflammatory microenvironment created in mice. (a) EphrinB2 mRNA levels were dramatically decreased in the pLenti6.3-efnb2siRNA group when compared with those in the pLenti6.3-ctrl group. (b) EphB4 mRNA levels were significantly downregulated in the pLenti6.3-ephb4siRNA group when compared with those in the pLenti6.3-ctrl group. (c–h) mRNA levels of bone-related genes such as Runx2 (c), Osx (d), ALP (e), OC (f), and BSP (g), as well as the osteoclastogenic transcription factor NFATc1 (h), were also monitored in the isolated newly formed bone tissue. Three independent experiments were performed in triplicate, and data were represented as mean ± SEM. , versus pLenti6.3-ctrl group.

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