Disturbed Expression of EphB4, but Not EphrinB2, Inhibited Bone Regeneration in an In Vivo Inflammatory Microenvironment

Figure 8

Immunohistochemistry staining for OC. (a–i) At 7 days (a–c), 14 days (d–f), and 21 days (g–i) after surgery, OC-positive staining could be observed in the osteoblasts, osteocytes, and bone matrix in the newly formed bone area in the pLenti6.3-ctrl group (a, d, g), the pLenti6.3-efnb2siRNA group (b, e, h), and the pLenti6.3-ephb4siRNA group (c, f, i). (j) Quantitative analysis via IOD measurements using the Image-Pro Plus 6.0 software. Arrow, positive OC staining; scale bars, 50 μm; < 0.05, < 0.001 versus pLenti6.3-ctrl group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)