Linking CD11b⁺ Dendritic Cells and Natural Killer T Cells to Plaque Inflammation in Atherosclerosis

Figure 2

Flow cytometry and gene expression results of leukocyte(s) (subsets) in ApoE^−/− mice during atherogenesis. Bar graphs representing mice sacrificed after 0 wk (white bars, ), 6 wk (grey bars, ), 12 wk (red bars, ), and 24 wk (blue bars, ) of WD. (a) Percentages of the total DC population. (b) Percentages of the CD11b⁺ cDC subset within the total DC population. (c) Percentages of CD103⁺ cDC within the total DC population. (d) Percentages of NKT cells within the total leukocyte population. (e–g) Graphs showing fluctuations (as percentage change over time) in T cells, Th cells, and monocytes/macrophages in blood (e), spleen (f), and mediastinal LN (g) at different time points (, , , and of WD). The arrow at time point 3 ( wk of WD) indicates dramatic leukocyte changes in all compartments. (h–k) Normalized expression levels of Zbtb46 (h), SIRPα (i), XCR1 (j), and Vα14-Jα18 (k) mRNA in aortic tissue samples; , , and .