2,3,5,4′-Tetrahydroxystilbene-2-O-β-glucoside Isolated from Polygoni Multiflori Ameliorates the Development of Periodontitis

Figure 3

THSG attenuated P. gingivalis LPS-induced proinflammatory cytokines expression via inducing SirT1 expression in HGFs. HGFs were cultured in 6-well plate and were starved with serum-free DMEM/F12 medium for 24 h when the cell confluence reached 80%. (a) HGFs were treated with 1 μg/mL P. gingivalis LPS, (b) 100 μM resveratrol, and (c) 100 μM THSG for 24 h. (d) HGFs were pretreated with resveratrol and THSG (1 and 25 μM) for 30 min and then were treated with 1 μg/mL P. gingivalis LPS for 6 h. The mRNA expression of SirT1 was examined by quantitative PCR. (;  ; ; were compared with control group. ; ; were compared with LPS group. ; were compared with 1 μM or 25 μM resveratrol group with LPS treatment.)

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