Exploring New Inflammatory Biomarkers and Pathways during LPS-Induced M1 Polarization

Figure 7

M1 polarized N9 microglia display upregulated levels of miR-155 and miR-146a, as well as downregulated miR-124, which are recapitulated in cell-derived exosomes. N9 cells were collected after incubation with lipopolysaccharide (LPS) and miR-155, miR-146a, and miR-124 expression were evaluated by qRT-PCR, both in cells (a) and in exosomes (c). Results are mean ± s.e.m. from five independent experiments. Comparisons between LPS-treated N9 cells and nontreated cells (control) were made via two-tailed Student’s -test. and versus nontreated cells (control). Vesicles isolated from the extracellular media of N9 cells were characterized in terms of their size by dynamic light scattering (b).

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