Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial Cells

Figure 2

Screening of SAA receptors in HAECs. qPCR analysis of 10 genes that encode known SAA receptors was conducted. HAECs were treated with recombinant human SAA, and total RNA was extracted at 0, 1, 3, and 6 h. Among the expression levels of SAA receptors, TLR2 mRNA expression induced by SAA was the highest and significantly higher than that in unstimulated HAECs. Each experiment was performed in triplicate. Values were the mean ± SD. , .