Serum Amyloid A Promotes E-Selectin Expression via Toll-Like Receptor 2 in Human Aortic Endothelial Cells

Figure 4

Effect of TLR2 siRNA on TLR2 expression and morphology in HAECs. The effect of TLR2 siRNA in HAECs was determined by qPCR analysis (a), western blotting (b), and observations of cell morphology (c). (a, b) mRNA and protein levels of TLR2 in HAECs were dramatically decreased by siRNA treatment for 6 h. (c) siTLR2 had no significant effect on the cell morphology of HAECs. Representative data of three independent mRNA experiments are shown. Each mRNA experiment was performed in triplicate. Values are the mean ± SD. . siCON: control siRNA; siTLR2: TLR2 siRNA.

(a) SAA (+)

(b) SAA (+)

(c)