Metallothioneins 1 and 2 Modulate Inflammation and Support Remodeling in Ischemic Cardiomyopathy in Mice
Impaired antioxidative control and maladaptation of contractile elements in M-hearts. RT-qPCR showing mRNA expression of (a) heme oxygenase (HMOX)1, (b) glutathione peroxidase (GPX)1, (c) superoxide dismutase (SOD)1, (d) SOD2, (e) SOD3, (f) Ras-related C3 botulinum toxin substrate (Rac)1, (g) peroxisome proliferator-activated receptor (PPAR)-α, (h) uncoupling protein (UCP) 3, (i) myosin heavy chain (MHC) isoform α, and (j) β-MHC isoform. (k) Quantification of cardiomyocyte size by area planimetry on collagen stained slides after 7 days of . = 8–10/group; RT-qPCR using Taqman, mRNA expression is related to shams and GAPDH using comparative ΔΔCt-method; indicates P ≤ 0.05 between the genotypes; # indicates P ≤ 0.05 versus respective sham.