Protective Effect of Adrenomedullin on Rat Leydig Cells from Lipopolysaccharide-Induced Inflammation and Apoptosis via the PI3K/Akt Signaling Pathway ADM on Rat Leydig Cells from Inflammation and Apoptosis

Figure 1

Effect of ADM on cell viability and LPS-induced damage of Leydig cells. Cell viability in different treatment groups was assessed using a CCK-8 assay. (a) Time course of effect of ADM (100 nM) on the viability of Leydig cells. (b) Dose-response effect of ADM (0, 10, 50, 100, or 300 nM) on the viability of Leydig cells after a 12-hour treatment. (c) ADM (0, 10, 50, 100, or 300 nM) was added for 2 h prior to a 12-hour treatment with LPS (1 μg/mL). Data were obtained from five independent experiments performed in triplicate and expressed as mean ± SEM.

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