(a)
(b)
(c)
Figure 1: Effect of ADM on cell viability and LPS-induced damage of Leydig cells. Cell viability in different treatment groups was assessed using a CCK-8 assay. (a) Time course of effect of ADM (100 nM) on the viability of Leydig cells. (b) Dose-response effect of ADM (0, 10, 50, 100, or 300 nM) on the viability of Leydig cells after a 12-hour treatment. (c) ADM (0, 10, 50, 100, or 300 nM) was added for 2 h prior to a 12-hour treatment with LPS (1 μg/mL). Data were obtained from five independent experiments performed in triplicate and expressed as mean ± SEM.