Protective Effect of Adrenomedullin on Rat Leydig Cells from Lipopolysaccharide-Induced Inflammation and Apoptosis via the PI3K/Akt Signaling Pathway ADM on Rat Leydig Cells from Inflammation and Apoptosis

Figure 3

Effect of ADM on LPS-induced gene expressions of IL-1, IL-6, iNOS, and COX-2 and the production of IL-1, IL-6, NO, and PGE2. Leydig cells were treated with ADM (100 nM) or ADM (100 nM) + LY294002 (10 μmol/L) for 2 h before a 12-hour treatment with LPS (1 μg/mL). The normalized levels of gene expression are expressed as ratios of the copy number of the mRNA and that of β-actin cDNA. Culture media were analyzed for nitrite concentration on behalf of NO production. Data were obtained from five independent experiments performed in triplicate and expressed as mean ± SEM.

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