Protective Effect of Adrenomedullin on Rat Leydig Cells from Lipopolysaccharide-Induced Inflammation and Apoptosis via the PI3K/Akt Signaling Pathway ADM on Rat Leydig Cells from Inflammation and Apoptosis

Figure 6

Effect of ADM on LPS-induced protein levels of Bcl-2, Bax, procaspase-3, cleaved caspase-3, PARP, and cleaved PARP and caspase-3 activity. Leydig cells were treated with ADM (100 nM) or ADM (100 nM) + LY294002 (10 μmol/L) for 2 h prior to a 12-hour treatment with LPS (1 μg/mL). Protein levels were detected by Western blot; caspase-3 activity was measured by spectrophotometry (OD, optical density). β-actin was used as internal reference. Data were obtained from five independent experiments performed in triplicate and expressed as mean ± SEM.

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