Mediators of Inflammation

Research Article

Protective Effect of Adrenomedullin on Rat Leydig Cells from Lipopolysaccharide-Induced Inflammation and Apoptosis via the PI3K/Akt Signaling Pathway ADM on Rat Leydig Cells from Inflammation and Apoptosis

Table 1

Sequences of primers for the real-time PCR experiments.
GeneAccession numberSenseSequence Size (bp)
IL-1NM_031512.2F
R		CATTGTGGCTGTGGAGAAG
ATCATCCCACGAGTCACAGA		130
IL-6NM_012589.1F
R		TCCTACCCCAACTTCCAATGCTC
TTGGATGGTCTTGGTCCTTAGCC		79
iNOSNM012611.3F
R		ACCAGTACGTTTGGCAATGG
TCAGCATGAAGAGCGATTTCT		70
COX-2NM017232.3F
R		CTTACAATGCTGACTATGGCTAC
AAACTGATGCGTGAAGTGCTG		242
Bcl-2NM001001280.1F
R		CCACCAAGAAAGCAGGAAACC
GGCAGGATAGCAGCACAGG		129
BaxNM017059.2F
R		TGGCAGCTGACATGTTTTCTGAC
CGTCCCAACCACCCTGGTCT		195
Caspase-3NM012922.2F
R		CGATTATGCAGCAGCCTCAA
AGGAGATGCCACCTCTCCTT		118
PARPNM013063.2F
R		TCTTTGATGTGGAAAGTATGAAGAA
GGCATCTTCTGAAGGTCGAT		64
β-actinNM031144.3F
R		CGTTGACATCCGTAAAGAC
TGGAAGGTGGACAGTGAG		201
F: forward; R: reverse.