Anti-Inflammatory Effect of Rhapontici Radix Ethanol Extract via Inhibition of NF-κB and MAPK and Induction of HO-1 in Macrophages

Table 1

Primers used for real-time RT-PCR.

Target gene	Primer sequence
TNF-α	F: 5′-TTCTGTCTACTGAACTTCGGGGTGATCGGTCC-3′
	R: 5′-GTATGAGATAGCAAATCGGCTGACGGTGTGGG-3′
IL-6	F: 5′-TCCAGTTGCCTTCTTGGGAC-3′
	R: 5′-GTGTAATTAAGCCTCCGACTTG-3′
IL-1β	F: 5′-ATGGCAACTGTTCCTGAACTCAACT-3′
	R: 5′-CAGGACAGGTATAGATTCTTTCCTTT-3′
iNOS	F: 5′-GGCAGCCTGTGAGACCTTTG-3′
	R: 5′-GCATTGGAAGTGAAGCGTTTC-3′
COX-2	F: 5′-TGAGTACCGCAAACGCTTCTC-3′
	R: 5′-TGGACGAGGTTTTTCCACCAG-3′
HO-1	F: 5′-TGAAGGAGGCCACCAAGGAGG-3′
	R: 5′-AGAGGTCACCCAGGTAGCGGG-3′
β-actin	F: 5′-AGAGGGAAATCGTGCGTGAC-3′
	R: 5′-CAATAGTGATGACCTGGCCGT-3′

F: forward; R: reverse.