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Mediators of Inflammation
Volume 2016, Article ID 7349603, 11 pages
Research Article

NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF-κB Signaling Pathway

1Department of Respiratory Medicine, General Hospital of Guangzhou Military Command of PLA, Guangzhou, Guangdong, China
2Department of Pulmonary Medicine, Chenzhou No. 1 People’s Hospital, Chenzhou, Hunan, China
3Department of Medical Intensive Care Unit, General Hospital of Guangzhou Military Command of PLA, Guangzhou, Guangdong, China
4Guangdong Provincial Key Laboratory of Geriatric Infection and Organ Function Support, Guangzhou, Guangdong, China

Received 11 July 2016; Revised 5 September 2016; Accepted 15 September 2016

Academic Editor: Yutong Zhao

Copyright © 2016 Jianhua Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of the present study is to investigate the protective effects and relevant mechanisms exerted by NEMO-binding domain peptide (NBD) against lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. The ALI model was induced by intratracheally administered atomized LPS (5 mg/kg) to BABL/c mice. Half an hour before LPS administration, we treated the mice with increasing concentrations of intratracheally administered NBD or saline aerosol. Two hours after LPS administration, each group of mice was sacrificed. We observed that NBD pretreatment significantly attenuated LPS-induced lung histopathological injury in a dose-dependent manner. Western blotting established that NBD pretreatment obviously attenuated LPS-induced IκB-α and NF-κBp65 activation and NOX1, NOX2, and NOX4 overexpression. Furthermore, NBD pretreatment increased SOD and T-AOC activity and decreased MDA levels in lung tissue. In addition, NBD also inhibited TNF-α and IL-1β secretion in BALF after LPS challenge. In conclusion, NBD protects against LPS-induced ALI in mice.