mTORC1-Activated Monocytes Increase Tregs and Inhibit the Immune Response to Bacterial Infections

Figure 1

E. coli infection activated mTORC1/S6k pathway in monocytes, and TSC1 KO mice partly simulated E. coli infection. (a) The percentage of EYFP in monocytes from Rosa-LysMCreTsc1 mice. This percentage represents the TSC1 deletion ratio in TSC1 KO monocytes. (b) The phosphorylation level of S6k in WT and TSC1 KO monocytes (CD11b⁺Ly6C⁺) in the presence or absence of Rapa (1.5 mg/kg, four days before treatment) (MFI: median fluorescence intensity) (three mice per group, three representative experiments). (c) The phosphorylation level of S6k in WT and TSC1 KO monocytes (CD11b⁺Ly6C⁺) with or without E. coli infection (0⁷ cells/mouse) for 8 h (three mice per group, three representative experiments). , , and compared to WT mice or between the indicated groups. values were determined using Student’s -tests.

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