mTORC1-Activated Monocytes Increase Tregs and Inhibit the Immune Response to Bacterial Infections

Figure 3

Change in cytokine expression by monocytes in mice with infected bacteria. Freshly isolated peritoneal monocytes (CD11b⁺Ly6C⁺) were sorted from WT, TSC1 KO, and TSC1 + Rapa (1.5 mg/kg, four days before treatment) mice after E. coli infection (0⁷ cells/mouse), and the expression of TNF-α, IFN-γ, IL-1β, IL-10, and TGF-β was determined by real-time PCR ( representative experiments). and compared to WT mice or between the indicated groups. values were determined using Student’s -tests.