mTORC1-Activated Monocytes Increase Tregs and Inhibit the Immune Response to Bacterial Infections

Figure 6

After E. coli infection, the expression of activators in monocytes decreased and the activation of effector T cells declined in TSC1 KO mice. (a) E. coli (ATCC, USA) were injected into each mouse through the tail vein for infection. Eight hours postinfection, the percentage and number of activated effector CD4+ T cells decreased in LNs and spl of TSC1 KO mice compared to WT mice. (b) E. coli (ATCC, USA) were injected into each mouse through the tail vein for infection. Eight hours postinfection, the expression levels of activators, including CD40, CD80, CD86, MHC-II, and CD14 in monocytes, decrease in TSC1 KO mice than WT mice. (c) 2 × 10⁵ naïve CD4⁺ T cells (CD4⁺CD4CD62⁺) were cocultured with 1 × 10⁵ sorted monocytes (CD11b⁺Ly6C⁺) from WT or TSC1 KO mice in DMEM with anti-mouse CD3e (10 μg/mL, BD Biosciences, USA) and CD28 antibody (10 μg/mL, BD Biosciences, USA) in 96-well flat-bottomed plates for 6 days. The proliferation of naïve CD4⁺ T cells cocultured with TSC1 KO monocytes was lower than cells cocultured with the WT group. and compared to WT mice or between the indicated groups. values were determined using Student’s -tests.

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