Small Interfering RNA Targeted to ASPP2 Promotes Progression of Experimental Proliferative Vitreoretinopathy

Figure 2

Knockdown of ASPP2 with siRNA efficiently inhibits ASPP2 expression. (a) ASPP2 mRNA expression was significantly downregulated in ARPE-19 cells 48 h after transfection, as measured by real-time RT-PCR. (b) The data of the relative ASPP2 protein in the NC, SC, and ASPP2-siRNA treatment group, normalized to -actin. (c) Representative western blot showing the decreased protein expression of ASPP2 in ARPE-19 cells with ASPP2-siRNA transfection. (d) Cells stained with antibody against ASPP2 (red) and cell nuclei with DAPI were examined by fluorescence microscopy. The fluorescence intensity represents the protein expression of ASPP2 in ARPE-19 cells. Data are shown as the mean ± SD, . versus NC or SC group. NC was set to 100%. NC, normal control cells; SC, scrambled control-siRNA-transfected cells; ASPP2-si, ASPP2-siRNA-transfected cells; DAPI, 4′,6′-diamino-2-phenylindole. Scale bars, 50 μm.

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