Small Interfering RNA Targeted to ASPP2 Promotes Progression of Experimental Proliferative Vitreoretinopathy

Figure 3

Cytotoxicity, proliferation, and migration induced by transfection of ARPE-19 cells. Normal control ARPE-19 cells without treatment and cells transfected with scrambled control-siRNA or ASPP2-siRNA were incubated for 48 h. Then, cells were stained with Annexin V and propidium iodide, followed by flow cytometry measurement. Rates of early apoptotic cells and late apoptotic cells are shown in the bottom right and top right quadrants, respectively (a, b, and c). Representative results of three independent experiments are shown. Cell proliferation was measured with a CCK-8 assay at 0, 12, 24, 48, and 72 h (d). Cell migratory activity was determined by Transwell assay 48 h after transfection (e). Statistical analysis based on the number of cells that had migrated through the filter of the chamber, showing that ASPP2 knockdown promoted cell migration significantly. Data are shown as the mean ± SD, experiments. versus normal control or Scrambled control-siRNA group.

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