Small Interfering RNA Targeted to ASPP2 Promotes Progression of Experimental Proliferative Vitreoretinopathy

Figure 4

Effects of ASPP2 on the protein expression of epithelial and mesenchymal markers of ARPE-19 cells. Western blot images of expression of epithelial markers (ZO-1, E-cadherin, and -catenin) were significantly decreased, while mesenchymal markers (-SMA, fibronectin, and ZEB1) significantly increased in ARPE-19 cells 48 h after ASPP2-siRNA transfection, when compared to the scrambled control-siRNA transfection (a, b, c, and d). -actin or -tubulin was used as a loading control. Representative results obtained in three independent experiments are shown. Column diagrams and bars represent the ratio of the scanned immunoblots of mesenchymal markers and epithelial markers to -actin or -tubulin. Data are shown as the mean ± SD, experiments. versus SC group; versus the SC group. SC was set to 100%. SC, scrambled control-siRNA-transfected cells; ASPP2-si, ASPP2-siRNA-transfected cells.

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