DNA Damage in CD133-Positive Cells in Barrett’s Esophagus and Esophageal Adenocarcinoma

Table 1

Immunoreactivity grading of CD133 among normal esophagus, Barrett’s esophagus, and Barrett’s adenocarcinoma tissues.

Factor	Group	^#IHC grade					⁢ value
		−	+	++	+++	++++	vs. Normal	vs. BE
CD133; Apical surface	Normal	7	0	0	0	0
	BE	4	5	4	6	0
	BEA	3	1	5	0	2
CD133; Cytoplasm and cell membrane	Normal	7	0	0	0	0
	BE	19	0	0	0	0
	BEA	4	4	1	2	0
8-oxodG and 8-nitroguanine double staining	Normal	6	1	0	0	0
	BE	0	4	7	7	1
	BEA	0	0	3	4	4

An IHC grade was assigned to each specimen according to the degree of staining as described in Section 2. Normal = normal esophagus, BE = Barrett’s esophagus, and BEA = Barrett’s esophageal adenocarcinoma.
⁢ values were calculated by chi-square test (versus (vs.) Normal and versus BE).
IHC grade was analyzed in normal mucosal, columnar, and cancer cells in normal esophageal, BE, and BEA tissues, respectively.