Periploca forrestii Saponin Ameliorates Murine CFA-Induced Arthritis by Suppressing Cytokine Production

Figure 5

Effect of Periplocin on proinflammatory cytokine expression in synoviocytes from normal Sprague Dawley rats. Synoviocytes were prepared as detailed in Methods. Synoviocytes were incubated with the indicated concentrations of Periplocin for 24 h in the presence or absence of LPS (1 μg/mL) stimulation. After 24 h, the mRNA was measured by RT-PCR and β-actin was used as the internal controls, respectively. Representative gels stained for RT-PCR products of IL-6 and TGF-β mRNA expression of synoviocytes in AA model rats treated with Periplocin. (a) RT-PCR analysis of inflammatory cytokine genes and related transcription factors and (b) relative amounts of each cytokine gene level were determined by densitometric analysis. Steady-state expression of β-actin was used to control equal loading of the PCR product onto gels. The histogram shows the mRNA levels. B: blank; L: LPS; P: Periplocin.

(a)

(b)