Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages

Figure 2

Characterization of M0, M1, and M2 polarized BMDMs. (a) Expression of polarization marker genes at the mRNA level (RT-qPCR) in M0, M1, and M2 macrophages. The expression of marker genes was analyzed as described in Figure 1 and the data were expressed as mean fold induction relatively to M0 cells ± SD (). (b) Expression of polarization markers at the protein level in M0, M1, and M2 macrophages. (b1) Production of secreted IL-6 (M1 marker) in cell culture supernatants assessed by ELISA. Data are expressed relatively per μg of protein per well as mean ± SD (). (b2) Expression of HO-1 and Srxn1 assessed by Western blotting in M0, M1, and M2 macrophages. Data are normalized with TBP used as loading control and expressed as mean ± SD (). Results are representative of 4 independent experiments. ANOVA 1: and .