Mediators of Inflammation / 2016 / Article / Fig 7

Research Article

Myeloperoxidase-Oxidized LDLs Enhance an Anti-Inflammatory M2 and Antioxidant Phenotype in Murine Macrophages

Figure 7

Comparative effects of LDLs on (un)polarized BMDMs to induce foam cells. (a) M0, M1, and M2 macrophages were incubated in the presence or absence of Nat-LDLs, Ox-LDLs, and MpOx-LDLs (100 μg/mL) for 24 hours. The number of lipid droplets was estimated using a High Content Imager, as described in Materials and Methods. Data are expressed as mean ± SD (). ANOVA 1: and . (b) Intracellular cholesterol content in M0, M1, and M2 macrophages incubated in the presence or absence of Nat-LDLs, Ox-LDLs, and MpOx-LDLs for 24 hours. The intracellular cholesterol was measured by a colorimetric assay (absorbance: 570 nm). Data are expressed as μg/μL of free, esterified, and total cholesterol (mean ± SD ()). Data were analyzed by a Kruskal-Wallis ANOVA on ranks. ANOVA 1: and .
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