ET-1 Promotes Differentiation of Periodontal Ligament Stem Cells into Osteoblasts through ETR, MAPK, and Wnt/β-Catenin Signaling Pathways under Inflammatory Microenvironment

Figure 7

Wnt/Ca²⁺ pathway was not involved in the differentiation of PDLSCs promoted by ET-1. RNA and proteins were extracted after PDLSCs had been treated with 10 nM ET-1 for 12 h, 24 h, and 72 h and 7 d, 14 d, and 21 d. And the levels of each mRNA (Dv1, IP3 kinase C, CaMK, NLK, and p38 MAPK) were measured. None of the above genes showed any change after ET-1 treatment (a), nor did the protein levels ((b) and (c)). These results indicated that Wnt/Ca²⁺ pathway was not involved in the differentiation of PDLSCs promoted by ET-1. Each bar represents the mean ± standard deviation ().

(a)

(b)

(c)