ET-1 Promotes Differentiation of Periodontal Ligament Stem Cells into Osteoblasts through ETR, MAPK, and Wnt/β-Catenin Signaling Pathways under Inflammatory Microenvironment

Table 1

The human primer sequences used for real-time PCR.

Target gene	Primers sequences		Size (bp)
Runx2	Forward	5′-TGGTTACTGTCATGGCGGGTA-3′	101
	Reverse	5′-TCTCAGATCGTTGAACCTTGCTA-3′
OCN	Forward	5′-CACTCCTCGCCCTATTGGC-3′	112
	Reverse	5′-CCCTCCTGCTTGGACACAAAG-3′
COL1	Forward	5′-GAGGGCCAAGACGAAGACATC-3′	140
	Reverse	5′-CAGATCACGTCATCGCACAAC-3′
GAPDH	Forward	5′-ACAACTTTGGTATCGTGGAAGG-3′	101
	Reverse	5′-GCCATCACGCCACAGTTTC-3′
ETRA	Forward	5′-TCGGGTTCTATTTCTGTATGCCC-3′	143
	Reverse	5′-TGTTTTTGCCACTTCTCGACG-3′
ETRB	Forward	5′-GTCCCAATATCTTGATCGCCAG-3′	132
	Reverse	5′-AAGGCACCAGCTTACACATCT-3′
ERK1/2	Forward	5′-CTACACGCAGTTGCAGTACAT-3′	157
	Reverse	5′-CAGCAGGATCTGGATCTCCC-3′
JNK	Forward	5′-TGTGTGGAATCAAGCACCTTC-3′	146
	Reverse	5′-AGGCGTCATCATAAAACTCGTTC-3′
p38	Forward	5′-CCCGAGCGTTACCAGAACC-3′	136
	Reverse	5′-TCGCATGAATGATGGACTGAAAT-3′
Axin	Forward	5′-GGTTTCCCCTTGGACCTCG-3′	157
	Reverse	5′-CCGTCGAAGTCTCACCTTTAATG-3′
APC	Forward	5′-AAGCATGAAACCGGCTCACAT-3′	108
	Reverse	5′-CATTCGTGTAGTTGAACCCTGA-3′
GSK3β	Forward	5′-GGCAGCATGAAAGTTAGCAGA-3′	180
	Reverse	5′-GGCGACCAGTTCTCCTGAATC-3′
β-catenin	Forward	5′-CCTATGCAGGGGTGGTCAAC-3′	95
	Reverse	5′-CGACCTGGAAAACGCCATCA-3′
TCF/LEF	Forward	5′-CGAAGGTCAAGCTATGAGGACA-3′	141
	Reverse	5′-ATCTGCGATGCTGGCAATCT-3′
Dv1	Forward	5′-GCAAACTCTCACTGTATAGGCAA-3′	152
	Reverse	5′-AGTACCATCAAATTCACGGGTC-3′
IP3 kinase C	Forward	5′-AGCCTGGAGAATTGCTGACTC-3′	181
	Reverse	5′-TCAGACTCGTCGAAAGAAGAGG-3′
CaMK	Forward	5′-ACCCGTTTCACCGACGACTA-3′	96
	Reverse	5′-CTCCTGCGTGGAGGTTTTCTT-3′
NLK	Forward	5′-CCAACCTCCACACATTGACTATT-3′	117
	Reverse	5′-ACTTTGACATGATCTGAGCTGAG-3′
P38 MAKP	Forward	5′-CTGTTGGACGTTTTTACACCTGC-3′	158
	Reverse	5′-AGACCTCGGAGAATTTGGTAGA-3′