Effect of Interferon-γ on the Basal and the TNFα-Stimulated Secretion of CXCL8 in Thyroid Cancer Cell Lines Bearing Either the RET/PTC Rearrangement Or the BRAF V600e Mutation

Figure 4

Representative images and the respective histograms of (a) BCPAP and (b) TPC-1 after 16 hours of migration within the transwell migration chamber system in different conditions. Nuclei were counterstained with Hoechst 33258 in blue (images were acquired by an Olympus BX51, magnification 20x, scale bar = 50 μm). Bar graphs show the corresponding analysis of migrated cells on the lower side of the transwell filter. BCPAP: ANOVA F = 58.71; ; post hoc analysis by Bonferroni: CXCL8 versus basal; IFNγ versus basal; IFNγ + CXCL8 versus IFNγ. TPC-1: ANOVA F = 8.85 ; post hoc analysis by Bonferroni: CXCL8 versus basal; NS IFNγ versus basal; NS IFNγ + CXCL8 versus IFNγ. versus basal; versus CXCL8 + IFNγ.

(a)

(b)