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Mediators of Inflammation
Volume 2016 (2016), Article ID 8543561, 12 pages
http://dx.doi.org/10.1155/2016/8543561
Research Article

Protective Effect of Amphipterygium adstringens Extract on Dextran Sulphate Sodium-Induced Ulcerative Colitis in Mice

1Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, 11400 Ciudad de México, Mexico
2Laboratorio de Inmunobiología, Carrera de Medicina, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico
3Laboratorio de Farmacognosia, UBIPRO, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico
4Unidad de Biomedicina, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, 54090 Tlalnepantla, MEX, Mexico

Received 18 March 2016; Accepted 3 July 2016

Academic Editor: Chang-Shik Yin

Copyright © 2016 Mario Rodriguez-Canales et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Amphipterygium adstringens is an endemic species in Mexico commonly known as “cuachalalate.” Healers to treat gastritis, gastric ulcers, and gastrointestinal cancer have traditionally used the bark. We investigated the effects of alcoholic extract of A. adstringens (AaEE) in DSS-induced colitis in mice. The protective effect of AaEE was determined at 200 mg/kg by oral gavage for 10 days. We determine the effect of AaEE on clinical features (disease activity index), antioxidants, anti-inflammatory, and immunomodulatory activities in relation to the activity of SOD, CAT, and GPx, levels of proinflammatory cytokines, and changes both macroscopic and microscopic of the colonic mucosa. AaEE significantly reduced the inflammation of colon and significantly increased SOD and GPx activities. AaEE also significantly decreased TNF-α, IFN-γ, and IL-1β cytokine levels compared to DSS-treated mice and reduced both infiltration of inflammatory cells and the mucosal damage in colon. The results suggested the protective potential of AaEE in DSS-induced colitis and this might be attributed to its phytochemicals compounds that have been found to induce a wide spectrum of activities such as reduction in oxidative stress, suppression of inflammation, modulating numerous signal transduction pathways, and induction of apoptosis. The findings of this study suggest that AaEE has substantial potential for the treatment of inflammatory colitis.