Novel Role of Endogenous Catalase in Macrophage Polarization in Adipose Tissue

Figure 1

Catalase deficiency accelerates oxidative stress, macrophage infiltration, and crown-like structures in epididymal WAT. (a) Immunohistochemistry for nitrotyrosine (1 : 200), a marker of oxidative stress. Scale bar, 50 μm; magnification, 100x. (b) F4/80 was assessed by real-time PCR and (c) immunohistochemistry (1 : 100) to measure macrophage infiltration. Immunostaining was quantified in Image Pro. Scale bar, 100 μm; magnification, 200x. (d) Crown-like structures in M1 macrophages were also quantified by immunohistochemistry for F4/80 (1 : 100, brown) and image analysis in Image Pro. Scale bar, 100 μm; magnification, 100x. Tissues were costained with hematoxylin (blue). Data are mean ± SE of 6–8 mice, and representative immunohistochemistry images are shown. .

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